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Composition, particle size and role in dietary fat transport of two different lipoproteins of the intestinal lymph
Author(s) -
Boquillon Micheline,
Paris Roger,
Clement Jacqueline
Publication year - 1972
Publication title -
lipids
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.601
H-Index - 120
eISSN - 1558-9307
pISSN - 0024-4201
DOI - 10.1007/bf02531512
Subject(s) - lymph , lipidology , chemistry , electrophoresis , chromatography , composition (language) , centrifugation , clinical chemistry , chylomicron , particle (ecology) , particle size , cholesterol , biochemistry , lipoprotein , biology , very low density lipoprotein , pathology , medicine , linguistics , philosophy , ecology
Rat intestinal lymph very low density lipoproteins have been separated by cellulose acetate electrophoresis. After administration of a meal including natural fats and labeled fatty acids, two labeled bands were detected on the electrophoregraphs. Further separations of these two kinds of particles were performed by density gradient zonal centrifugation. The isolated lipoproteins were analyzed for chemical composition and investigated by electron microscopy and electrophoresis. The moving particles had a higher protein and cholesterol content and a smaller diameter than the particles which remained at the origin. It has also been shown that the major part of the labeled lymph lipids were transported by the smaller particles.