Premium
The metabolism of 1‐ 14 C‐palmitic acid in the testis of the rat
Author(s) -
Coniglio J. G.,
Bridges R. B.,
Aguilar H.,
Zseltvay R. R.
Publication year - 1972
Publication title -
lipids
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.601
H-Index - 120
eISSN - 1558-9307
pISSN - 0024-4201
DOI - 10.1007/bf02531505
Subject(s) - palmitic acid , triglyceride , lipidology , fatty acid , clinical chemistry , chemistry , metabolism , incubation , stearate , catabolism , biochemistry , endocrinology , medicine , biology , cholesterol , organic chemistry
The metabolism of 1‐ 14 C‐palmitic acid was studied in rat testis at various intervals after intratesticular injection and after a 2 hr incubation period. Significant catabolism occurred as evidenced by production of 14 CO 2 in both in vivo and in vitro experiments. Of the 14 C activity retained in the injected testis, less than 15% remained as free fatty acid after 2 hr and less than 5% by the end of 2 weeks. Activity of 14 C appeared in phosphatides faster than in the triglyceride fraction, and with time (1–2 weeks) the activity in phosphatides decreased relative to that in triglycerides. In phosphatides of these experiments ca. 80% of the 14 C was in the palmitic acid fraction and the balance predominantly (98%) in palmitate with the balance in stearate and oleate. By the first and second weeks almost half of the 14 C‐palmitate present in the testes had been newly synthesized from 14 C‐acetyl CoA resulting from oxidation of the administered 1‐ 14 C‐palmitate. In the incubated samples the only fatty acid with 14 C activity in any lipid fraction was palmitate. In these experiments ca 90% of the 14 C was in the free fatty acid fraction, 7% in the phosphatides and 2% in the triglycerides.