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The effect of sonication on lipase activity
Author(s) -
Goodman L. P.,
Dugan L. R.
Publication year - 1970
Publication title -
lipids
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.601
H-Index - 120
eISSN - 1558-9307
pISSN - 0024-4201
DOI - 10.1007/bf02531470
Subject(s) - sonication , tripalmitin , lipase , chemistry , triacylglycerol lipase , chromatography , hydrolysis , substrate (aquarium) , biochemistry , enzyme , biology , ecology
Studies were made to evaluate the effect of sonication on activity of porcine pancreatic lipase and on activity of this lipase when in contact with substrate. Lipase was rapidly inactivated by sonication at 50 C, but was stable at 30 C or lower temperatures. Sonication for 4.5 min of lipase and olive oil at 38 C produced 2.7 times as much free fatty acid as without sonication. Hydrolysis of tripalmitin was achieved by sonication of lipase and tripalmitin dissolved in methyl myristate.