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Identification and characterization of fully deuterated fatty acids isolated from Scenedesmus obliquus cultured in deuterium oxide
Author(s) -
Graff Gustav,
Szczepanik Patricia,
Klein Peter D.,
Chipault J. R.,
Holman Ralph T.
Publication year - 1970
Publication title -
lipids
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.601
H-Index - 120
eISSN - 1558-9307
pISSN - 0024-4201
DOI - 10.1007/bf02531394
Subject(s) - chemistry , deuterium , fatty acid , gas chromatography , nuclear magnetic resonance spectroscopy , spectroscopy , mass spectrometry , chromatography , organic chemistry , transmethylation , biochemistry , amino acid , physics , quantum mechanics , methionine
The alga Scenedesmus obliquus cultured in deuterated water, synthesized fully deuterated saturated and unsaturated long chain fatty acids. Their methyl esters were purified and their equivalent chain lengths were determined by gas liquid chromatography (GLC). They were characterized by mass spectroscopy, IR and near‐IR spectroscopy, and NMR spectroscopy. Hexadeca‐3,6‐dienoic acid was identified. The fatty acid compositions of the total lipid and of individual lipid classes were measured. The melting point of methyl perdeuteriohexadecanoate was lower than that of its hydrogen counterpart. Methyl esters of perdeuterio fatty acids had shorter retention times in GLC chromatography on polar and nonpolar phases.