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Two dimensional thin layer chromatographic separation of polar lipids and determination of phospholipids by phosphorus analysis of spots
Author(s) -
Rouser George,
Fleischer Sidney,
Yamamoto Akira
Publication year - 1970
Publication title -
lipids
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.601
H-Index - 120
eISSN - 1558-9307
pISSN - 0024-4201
DOI - 10.1007/bf02531316
Subject(s) - chromatography , lipidology , thin layer chromatography , chemistry , spots , polar , resolution (logic) , phospholipid , phosphorus , lipidomics , membrane , thin layer , layer (electronics) , analytical chemistry (journal) , clinical chemistry , biochemistry , organic chemistry , physics , astronomy , artificial intelligence , computer science
Separation of polar lipids by two‐dimensional thin layer chromatography providing resolution of all the lipid classes commonly encountered in animal cells and a sensitive, rapid, reproducible procedure for determination of phospholipids by phosphorus analysis of spots are described. Values obtained for brain and mitochondrial inner membrane phospholipids are presented.