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Association of phospholipid‐cholesterol micelles with rat heart mitochondria: Stimulators and inhibitors
Author(s) -
Dobiasova Milada,
Linhart Jaroslav
Publication year - 1970
Publication title -
lipids
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.601
H-Index - 120
eISSN - 1558-9307
pISSN - 0024-4201
DOI - 10.1007/bf02531306
Subject(s) - chemistry , lecithin , phospholipid , biochemistry , phosphatidylethanolamine , mitochondrion , cholesterol , divalent , liposome , sphingomyelin , phosphatidylcholine , chromatography , membrane , organic chemistry
Abstract The uptake of emulsified labeled lipids by rat heart mitochondria was studied. Divalent cations greatly stimulated uptake of cholesterol in emulsions containing phospholipids; the effect increased with increasing atomic number. Epinephrine and norepinephrine were also stimulatory. Lipid‐depleted mitochondria were less effective in taking up cholesterol unless lecithin or serum lipids were included in the emulsion. Addition of iodoacetamide did not inhibit uptake, while use of heated mitochondria or extremes of pH augmented lipid uptake. Thus the process appears to be non‐enzymatic. While lecithin emulsions showed no visible change on addition of calcium ions, phosphatidylethanolamine emulsions became turbid and the turbidity was largely removed by addition of heparin. Heparin as well as chondroitin sulfate B and nonionic detergent did inhibit lipid uptake by mitochondria. The possible role of such nonenzymatic lipid uptake in membrane formation and cholesterol accumulation is discussed.