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Stimulation in vitro of galactocerebroside galactosidase by N‐decanoyl 2‐amino‐2‐methylpropanol
Author(s) -
Arora R. C.,
Radin N. S.
Publication year - 1972
Publication title -
lipids
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.601
H-Index - 120
eISSN - 1558-9307
pISSN - 0024-4201
DOI - 10.1007/bf02531270
Subject(s) - stimulation , galactocerebroside , chemistry , ceramide , sphingosine , clinical chemistry , enzyme , biochemistry , substrate (aquarium) , in vitro , stereochemistry , medicine , receptor , biology , apoptosis , ecology , myelin , oligodendrocyte , central nervous system
Amides resembling ceramide (fatty acyl sphingosine) were synthesized and tested in vitro for their effects on the rat brain β‐galactosidase which hydrolyzes galactosyl ceramide. The N‐decanoyl derivative of 2‐amino‐2‐methyl‐1‐propanol was most effective, giving a 34% stimulation at 0.15 mM concentration and a 60% stimulation at maximal levels. Addition of a hydroxyl group in the 3 position reduced the degree of stimulation, as did increasing or decreasing the length of the fatty acid portion. Omission of the branched methyl group resulted in inhibition instead of stimulation. Kinetic analysis indicates that the stimulator does not affect the binding of substrate to enzyme, but does speed the rate of hydrolytic action. Stimulation was also observed with the cerebrosidase in spleen and kidney. It is suggested that the stimulators act on an enzyme site other than the substrate‐active site.