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The effects of biliary obstruction on the rates of uptake, synthesis and secretion of 14 C‐labeled cholesterol by isolated perfused rat livers
Author(s) -
Ferris R. M.,
Jacobj H. P.,
Barak A. J.
Publication year - 1972
Publication title -
lipids
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.601
H-Index - 120
eISSN - 1558-9307
pISSN - 0024-4201
DOI - 10.1007/bf02531264
Subject(s) - cholesterol , medicine , endocrinology , lipidology , secretion , clinical chemistry , metabolism , in vitro , biology , chemistry , biochemistry
The isolated perfused rat liver technique was utilized to study the effect of biliary obstruction on the rates of uptake, synthesis and secretion of 14 C‐labeled cholesterol in an effort to elucidate the mechanism of hypercholesterolemia in this condition. By perfusing normal and biliary obstructed livers with a medium containing 14 C‐labeled acetate or 14 C‐labeled lipoprotein cholesterol and by measuring cholesterol biosynthesis in vitro, it was possible to clarify some of the questions concerning these aspects of lipid metabolism. The results of these studies revealed that the uptake of cholesterol was not altered in biliary obstruction. Instead the hypercholesterolemia of biliary obstruction arises from an increased rate of secretion of cholesterol from liver, which is accompanied by an increased rate of hepatic cholesterolgenesis. Evidence is also presented to suggest that a substance is present in biliary obstructed blood which is capable of initiating increased cholesterolgenesis in normal livers.