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Fatty acid specificity of glyceride synthesis by homogenates of bovine mammary tissue
Author(s) -
Askew E. W.,
Emery R. S.,
Thomas J. W.
Publication year - 1971
Publication title -
lipids
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.601
H-Index - 120
eISSN - 1558-9307
pISSN - 0024-4201
DOI - 10.1007/bf02531204
Subject(s) - glyceride , stearic acid , oleic acid , fatty acid , palmitic acid , chemistry , biochemistry , linoleic acid , triglyceride , degree of unsaturation , chromatography , organic chemistry , cholesterol
Fatty acid esterification by cell free preparations of bovine mammary tissue was investigated to determine if the type of long chain fatty acid supplied might influence the rate of triglyceride synthesis by that tissue. Homogenates of lactating bovine mammary tissue esterified 14 C‐fatty acids into glycerides at rates dependent upon chain length and degree of unsaturation. Palmitic, stearic, oleic and linoleic acids were esterified at rates consistent with their concentration in milk fat. A comparison of free fatty acid concentrations of mammary tissue with levels saturating esterification suggested that supply of fatty acids does not limit glyceride synthesis. Certain combinations of fatty acids were facilitory, competitive or inhibitory to esterification. Stearic acid complimented esterification of palmitic and oleic acids. Unlabeled trans ‐11‐octadecenoic acid did not compete with 14 C‐palmitate as efficiently in the esterification process as did unlabeled cis ‐9‐octadecenoic acid, indicating that the mammary gland may preferentially esterify the cis ‐isomer of C‐18∶1. Linoleic acid inhibited esterification of palmitic, stearic and oleic acids.