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Lipid composition and endogenous respiration of pig heart mitochondria
Author(s) -
Comte J.,
Gautheron D.,
Peypoux F.,
Michel G.
Publication year - 1971
Publication title -
lipids
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.601
H-Index - 120
eISSN - 1558-9307
pISSN - 0024-4201
DOI - 10.1007/bf02531169
Subject(s) - cardiolipin , biochemistry , oxidative phosphorylation , mitochondrion , chemistry , phospholipid , endogeny , respiration , composition (language) , hexokinase , clinical chemistry , lipidology , metabolism , biology , glycolysis , membrane , botany , linguistics , philosophy
Lipid composition and endogenous respiration of pig heart mitochondria were studied in parallel, since the level of endogenous respiration affects the oxidation of added substrates and therefore the regulation of oxidative phosphorylation; mitochondrial lipids can interfere either as substrates or as partner in the energy conservation mechanism. O 2 uptake kinetics were measured in presence of different additives: ATP, ADP, NAD + and hexokinase + glucose. The lipid composition of pig heart mitochondria was determined by chromatographic and spectrophotometric methods. Total lipids were 90% phospholipids; the main phosphatides were cardiolipin, phosphatidyl choline and phosphatidyl ethanolamine; the two latter were rich in plasmalogens. The main nonpolar lipids were triglycerides and free fatty acids. The fatty acid composition of total lipids, phospholipids, free fatty acids and triglycerides was determined by gas liquid chromatography. Mitochondrial lipids were characterized by a high content of unsaturation.