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Cholesterol metabolism and vitamin B 6 : II. Intestinal cholesterogenesis in vitamin B 6 deficient rats
Author(s) -
Avery M. D.,
Lupien P. J.
Publication year - 1970
Publication title -
lipids
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.601
H-Index - 120
eISSN - 1558-9307
pISSN - 0024-4201
DOI - 10.1007/bf02531104
Subject(s) - ileum , medicine , vitamin , jejunum , cholesterol , endocrinology , lipidology , clinical chemistry , chemistry , metabolism , sodium , biochemistry , biology , organic chemistry
The in vitro incorporation of 1‐ 14 C‐acetate into cholesterol by vitamin B 6 deficient and pair‐fed rat intestinal tissue was assayed. It was found that vitamin B 6 deficiency increases, by a twofold factor, the incorporation of 1‐ 14 C‐acetate into the cholesterol biosynthetic chain, when using a segment of the ileum, and on the other hand no increase was observed when using a segment from the jejunum. The production of 14 CO 2 from 1‐ 14 C‐acetate was not found altered by a vitamin B 6 deficiency when using segments from the jejunum or the ileum. The effect of sodium cholate and sodium chenodeoxycholate on the incorporation of radioactivity into cholesterol and on the production of radioactive CO 2 , with 1‐ 14 C‐acetate as a substrate, was assayed with the two groups of animals. The significance of these findings and the posible relationships between these factors are discussed.