Premium
Relation of cholanate structure to inhibition of 14 C‐26‐cholesterol oxidation by mitochondria from rat liver
Author(s) -
Inkpen J. A.,
Inkpen C. A.,
Quackenbush F. W.
Publication year - 1970
Publication title -
lipids
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.601
H-Index - 120
eISSN - 1558-9307
pISSN - 0024-4201
DOI - 10.1007/bf02531098
Subject(s) - taurine , hydroxylation , glycine , mitochondrion , chemistry , conjugate , cholesterol , in vitro , inhibitory postsynaptic potential , biochemistry , clinical chemistry , stereochemistry , amino acid , enzyme , biology , endocrinology , mathematical analysis , mathematics
Eight bile acids and 20 of their derivatives, of known purity, were compared for inhibitory effect upon oxidation of cholesterol in vitro by rat liver mitochondria. All inhibited oxidation of 14 C‐26‐cholesterol; none inhibited oxidation of 14 C‐1‐octanoate. The α‐mono‐hydroxy‐ and α‐dihydroxycholanic acids were more potent inhibitors than α‐trihydroxycholanic acid and trioxocholanic acid. Most were more potent than their derivatives. In general, the relative inhibitory potency of derivatives was: methyl esters > free alcohols > glycine conjugates > taurine conjugates. Mitochondria from rats subjected to 4 hr alternate light‐dark periods were less susceptible to the inhibitory action of cholanate conjugates than were mitochondria from rats under normal day‐night conditions. These experiments with compounds of known purity show that the hydroxylation pattern is the determining factor in cholanate inhibition of the cholesterol oxidation.