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Ceramide phosphorylglycerol phosphate a new sphingolipid found in bacteria
Author(s) -
White David C.,
Tucker Anne N.
Publication year - 1970
Publication title -
lipids
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.601
H-Index - 120
eISSN - 1558-9307
pISSN - 0024-4201
DOI - 10.1007/bf02531095
Subject(s) - ceramide , sphingolipid , biochemistry , cardiolipin , chemistry , phosphorylcholine , sphingosine , phosphatidylglycerol , amide , sphingomyelin , biology , stereochemistry , phospholipid , phosphatidylcholine , membrane , apoptosis , receptor
Ceramide phosphorylglycerol phosphate (CPGP) has been identified in the lipid extract of the anaerobic bacterium Bacteroides melaninogenicus . To our knowledge this is the first report of this lipid in biological material. The ceramide derivative contains two phosphates, an amide linked fatty acid and a dihydrosphingosine long chain base. Glycerol diphosphate (PGP) identified by paper and column chromatography can be isolated after mild acid hydrolysis of the ceramide derivative. Inorganic phosphate is liberated quantitatively on treatment of the PGP from the ceramide derivative with alkaline phosphatase. The proportions of the fatty acids found linked to the amide of the dihydrosphingosine (LCB) differ from those esterified to cardiolipin in this organism. The long chain base appears to consist of part of an homologous series of branched and normal LCB containing from 17 to 21 carbon atoms. Previous work has indicated that ceramide phosphorylethanolamine and ceramide phosphorylglycerol (CPG) are present in the lipid extracts of B. melaninogenicus . By analogy with phosphatidylglycerol synthesis, CPGP is postulated to be an intermediate in the synthesis of CPG.