Premium
Biosynthesis of glycolipids: Incorporation of N‐acetyl galactosamine by a rat brain particulate preparation
Author(s) -
Handa Shizuo,
Burton Robert M.
Publication year - 1969
Publication title -
lipids
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.601
H-Index - 120
eISSN - 1558-9307
pISSN - 0024-4201
DOI - 10.1007/bf02531046
Subject(s) - glycolipid , biosynthesis , galactosamine , chemistry , ceramide , enzyme , biochemistry , transferase , glucosamine , substrate (aquarium) , stereochemistry , clinical chemistry , glycosyltransferase , ganglioside , lipidology , biology , apoptosis , ecology
A mixture of UDP‐(N‐acetyl)glucosamine‐1 14 C and UDP‐(N‐acetyl)galactosamine‐1 14 C (7∶3) served as a substrate to demonstrate in young rat brain tissue the presence of enzymes which catalyzes the reactions: UDP‐glcNAc⇌UDP‐galNAc (E.C.5.1.3) and UDP‐galNAc+ gal‐glc‐cer→ galNAc‐gal‐glc‐cer+UDP. The glycolipid acceptor specificity was examined and preliminary kinetic data were obtained for the UDP‐(N‐acetyl)galactosamine: gal‐glc‐ceramide N‐acetyl galactosamine transferase. The products of the reaction were identified. The relationship of this reaction to ganglioside biosynthesis is discussed.