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Stereospecific analysis of the major triglyceride species in the monounsaturated fraction of cocoa butter
Author(s) -
Sampugna J.,
Jensen R. G.
Publication year - 1969
Publication title -
lipids
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.601
H-Index - 120
eISSN - 1558-9307
pISSN - 0024-4201
DOI - 10.1007/bf02531021
Subject(s) - geotrichum , interesterified fat , chemistry , triglyceride , lipase , fraction (chemistry) , oleic acid , monoglyceride , digestion (alchemy) , chromatography , glyceride , organic chemistry , food science , fatty acid , biochemistry , enzyme , cholesterol
A stereospecific analysis employing pancreatic lipase, Geotrichum candidum lipase and phospholipase A was applied to the monounsaturated triglyceride fraction of cocoa butter. Diacid triglycerides required additional analyses involving the gas liquid chromatographic determination of the proportions of digly ceride acetates produced by separately acetylating the α,α and α,β‐digly cerides obtained from a G. candidum digestion mixture. Results indicated that the major triacid triglyceride in cocoa butter was rac glyceryl‐l‐palmitate‐2‐oleate‐3‐stearate. Approximately 5% of cocoa butter was comprised of triglycerides which were solely or predominantly of one enantiomer, containing oleic acid in the 3 position.