Premium
Cholesterol content of human parotid saliva
Author(s) -
Jacobsohn Gert M.,
Montiel Raf Ael J.
Publication year - 1969
Publication title -
lipids
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.601
H-Index - 120
eISSN - 1558-9307
pISSN - 0024-4201
DOI - 10.1007/bf02531009
Subject(s) - chromatography , saliva , chemistry , densitometry , cholesterol , residue (chemistry) , ethanol , gas chromatography , thin layer chromatography , ether , lipidology , biochemistry , medicine , organic chemistry
Free and esterifed cholesterol were determined in parotid fluid collected from 19 individuals by encapsulation of Stenson's duct in the oral 16.7±7.1 (SD) μg/100 ml and cholesterol esters averaged 13.2±8.0 (SD) μg/100 ml. Two assays are described for the measurement of cholesterol in parotid saliva. Lipophilic material was extracted with ether‐ethanol (3∶1). In assay 1, the solid residue of the extract was separated into its components by thin layer chromatography. Substances on the chromatogram were made to fluoresce and the spots were photographed. Cholesterol was determined by densitometry of its black image on film. In assay 2, the ether‐ethanol residue was subjected to quantitative gas chromatography. Both methods yield similar data.