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The role of lipids in membrane transport in Mycoplasma laidlawii
Author(s) -
Smith Paul F.
Publication year - 1969
Publication title -
lipids
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.601
H-Index - 120
eISSN - 1558-9307
pISSN - 0024-4201
DOI - 10.1007/bf02531002
Subject(s) - biochemistry , chemistry , metabolism , glycerol , membrane , diglyceride , clinical chemistry , membrane lipids , fatty acid
Pulse labeling of metabolizing cells of Mycoplasma laidlawii , strain B with 14 C‐glucose results in rapid turnover of the glucose moiety in carotenyl glucoside and phosphatidyl glucose. Pulse labeling also occurs in the fatty acid radicals of carotenyl esters. No labeling of phosphatidyl glycerol, carotenol or monoglucosyl diglyceride occurs. No turnover of 14 C occurs in diglucosyl diglyceride. Rapid turnover of 32 P in phosphatidyl glucose, but not in phosphatidyl glycerol, also occurs. The rate of 14 CO 2 evolution is equivalent to the rate of disappearance of 14 C in total lipids of cells prelabeled with 14 C‐glucose. β‐Glucosidase inhibition results in inhibition of glucose utilization. The reverse effect is not seen. A theory involving lipids as carriers in the transport of glucose into the cell and acetate, the end product of glucose metabolism, out of the cell is presented. This theory is compatible with known information concerning membrane structure, membrane transport and metabolism in Mycoplasmas.