Premium
The lipids of human pancreas with special reference to the presence of fatty acid methyl esters
Author(s) -
Lough A. K.,
Garton G. A.
Publication year - 1968
Publication title -
lipids
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.601
H-Index - 120
eISSN - 1558-9307
pISSN - 0024-4201
DOI - 10.1007/bf02530932
Subject(s) - chemistry , palmitic acid , fatty acid , fatty acid methyl ester , methanol , oleic acid , linolenic acid , chromatography , clinical chemistry , lipidology , chloroform , organic chemistry , extraction (chemistry) , biochemistry , benzene , linoleic acid , catalysis , biodiesel
Total lipids were extracted from human pancreas with chloroform‐methanol, chloroform‐methanol following acidification, and benzene. A similar proportional amount of total lipid was obtained by each procedure. Regardless of the method of extraction (i.e., whether or not methanol was present), a small proportion (about 1%) of the total lipid was found to consist of fatty acid methyl esters. Triglycerides constituted the major fraction (about 80%) of the pancreatic lipids; in addition to methyl esters, the remaining lipids comprised free fatty acids, phospholipids, cholesterol esters, and traces of free cholesterol. In general, each class of lipid had a similar over‐all fatty acid composition with palmitic and oleic acids as predominant components. The methyl esters had a relatively high content of linolenic acid, and the free fatty acids contained a notably high proportion of palmitic acid, in each case accompanied by a corresponding decrease in the proportion of oleic acid present.