Premium
Ultracentrifugal isolation of serum chylomicron‐containing fractions with quantitation by infrared spectrometry and NCH elemental analysis
Author(s) -
Hatch F. T.,
Freeman N. K.,
Jensen L. C.,
Stevens G. R.,
Lindgren F. T.
Publication year - 1967
Publication title -
lipids
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.601
H-Index - 120
eISSN - 1558-9307
pISSN - 0024-4201
DOI - 10.1007/bf02530920
Subject(s) - chylomicron , chemistry , chromatography , population , mass spectrometry , lipoprotein , biochemistry , very low density lipoprotein , cholesterol , demography , sociology
An ultracentrifugal method for isolating chylomicron‐containing fractions from serum by flotation, using either standard Spinco swinging‐bucket rotors or a specially fabricated swinging‐bucket rotor, is described. Lower limits of the S f rates of the chylomicron fractions are evaluated using a computer technique to define lipoprotein flotation over a nonlinear NaCl density gradient. The latter is prepared by a special overlayering technique. Quantitation within a 9–50 μg region of mass assay is accomplished by both infrared spectrometry and elemental analysis for N, C and H. Results indicate that the chylomicron concentration in serum for a small population of nonfasting male adults ranges from approximately 0–50 mg %.