Premium
Determination of the structure of lecithins via the formation of acetylated 1,2‐diglycerides
Author(s) -
Privett O. S.,
Nutter L. J.
Publication year - 1967
Publication title -
lipids
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.601
H-Index - 120
eISSN - 1558-9307
pISSN - 0024-4201
DOI - 10.1007/bf02530915
Subject(s) - chemistry , lipidology , chromatography , diglyceride , clinical chemistry , acetylation , organic chemistry , polymer science , food science , biochemistry , fatty acid , gene
Abstract A detailed procedure for quantitative determinations of molecular species of lecithins is described and applied to several lecithins isolated from natural sources. The method is based on the conversion of lecithin to acetylated 1,2‐diglycerides and analysis of these compounds by methodology used for the determination of triglyceride structure. The preparation of the acetylated 1,2‐diglycerides was carried out via hydrolysis with phospholipase C and acetylation of the resultant, 1,2‐diglycerides with pyridine‐acetic anhydride. Preparation of acetylated 1,2‐diglycerides from lecithin by acetolysis with acetic acid‐acetic anhydride was shown to be accompanied by intermolecular as well as intramolecular rearrangement of the fatty acids. The structure of the acetylated 1,2‐diglycerides was determined by a combination of argentation‐TLC and pancreatic lipase hydrolysis using internal standards for quantification. The method was applied to lecithins isolated from milk serum, egg, soybean, safflower seed and wheat germ lipids.