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Quantitative extraction of hamster liver lipid and cholesterol with supercritical carbon dioxide
Author(s) -
Kahlon Talwinder S.,
Chow Faye I.,
Sayre Robert N.
Publication year - 1996
Publication title -
journal of the american oil chemists' society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.512
H-Index - 117
eISSN - 1558-9331
pISSN - 0003-021X
DOI - 10.1007/bf02525466
Subject(s) - supercritical carbon dioxide , chemistry , chromatography , cholesterol , chloroform , supercritical fluid , extraction (chemistry) , methanol , hamster , supercritical fluid extraction , ethanol , biochemistry , organic chemistry , biology , endocrinology
A quantitative method for liver lipid and cholesterol extraction with supercritical CO 2 and ethanol entrainer (SCE) is reported and compared with the Folch (chloroform/methanol) procedure. Mean values for lipid and cholesterol in hamster livers ( n =48) were similar between the SCE and Folch methods. Correlation coefficients between the two methods were 0.9866 for total lipid and 0.9546 for cholesterol. Similar mean values and high correlations between the two methods validate the SCE procedure as a precise alternative method for quantitative liver lipid extractions. The SCE method also reduces the use of hazardous solvents.