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Formation and characterization of liposomes from lipid/proteic material extracted from pig stratum corneum
Author(s) -
Lopez O.,
Maza A.,
Coderch L.,
Parra J. L.
Publication year - 1996
Publication title -
journal of the american oil chemists' society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.512
H-Index - 117
eISSN - 1558-9331
pISSN - 0003-021X
DOI - 10.1007/bf02523916
Subject(s) - pulmonary surfactant , liposome , chemistry , sonication , stratum corneum , chromatography , sodium dodecyl sulfate , vesicle , phosphatidylcholine , permeability (electromagnetism) , chloroform , membrane , phospholipid , biochemistry , medicine , pathology
The formation and physicochemical properties of liposomes prepared from a mixture of lipids and proteins extracted from pig stratum corneum have been investigated. The extraction of this material was carried out with chloroform/methanol mixtures. The sonication of these mixtures at 80°C in water that contained piperazine‐1,4‐ bis (2‐ethanesulfonic acid) led to the formation of bilayered structures (vesicle size of about 150 nm), which were stable to aggregation for more than 24 h. The interaction of these liposome suspensions (proteoliposomes) at a subsolubilizing level with surfactants indicates that the nonionic surfactant Triton X‐100 had the largest capacity for altering liposome permeability, whereas the amphoteric surfactant dodecyl betaine exhibited the smallest. The anionic surfactant sodium dodecyl sulfate showed an intermediate activity relative to that shown by the other surfactants tested. Despite the fact that the proteoliposomes showed negligible permeability in the absence of surfactants, compared with that of phosphatidylcholine liposomes, addition of identical amounts of surfactants resulted after 45 min in similar permeability effects for both. However, the proteoliposomes appeared to be more resistant to the action of surfactants in the initial interaction period.