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A detailed triglyceride analysis of Lesquerella fendleri oil: Column chromatographic fractionation followed by supercritical fluid chromatography
Author(s) -
Hayes Douglas G.,
Kleiman Robert
Publication year - 1996
Publication title -
journal of the american oil chemists' society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.512
H-Index - 117
eISSN - 1558-9331
pISSN - 0003-021X
DOI - 10.1007/bf02523908
Subject(s) - fractionation , triglyceride , chromatography , chemistry , column chromatography , supercritical fluid , supercritical fluid chromatography , lipolysis , organic chemistry , cholesterol , biochemistry , adipose tissue
The triglyceride structure of oil from Lesquerella fendleri , a potential new U.S. crop, rich in C 20 hydroxy fatty acids, was examined by silica gel column chromatographic fractionation followed by supercritical fluid chromatography. The analysis confirmed previous findings derived by our research group, but provided further detail. The analysis demonstrated the presence of trihydroxy triglyceride, which contained all of the oil’s C 18 hydroxy acyl groups (present at less than 0.5% in the oil). Lipolysis indicated that these groups were located solely at the 2‐position. In addition, a strong correlation was detected between the presence of α‐linolenic (18:3 9,12,15 ) and auricolic (20:2 11,17 OH 14 ) acids in triglycerides.