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Quick method for estimating free gossypol in cottonseed, meats, collets, and extracted meals
Author(s) -
Hron R. J.,
Kuk M. S.,
Wan P. J.
Publication year - 1996
Publication title -
journal of the american oil chemists' society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.512
H-Index - 117
eISSN - 1558-9331
pISSN - 0003-021X
DOI - 10.1007/bf02523895
Subject(s) - absorbance , acetone , chromatography , bottle , analytical chemistry (journal) , mathematics , gossypol , chemistry , correlation coefficient , sample (material) , coefficient of determination , cottonseed , partial least squares regression , statistics , food science , materials science , organic chemistry , biochemistry , composite material
A method for estimating free gossypol (FG) has been developed that decreases sample‐determination time from over 2 h to about 25 min per sample. With auto pipetters and bottle‐to dispensers, six sample determinations can be completed in approximately 50 min. The method consisted of adding water and acetone separately to a fixed sample weight, mixing, filtering, diluting with 65% acetone, and reading absorbance on a spectrophotometer. Absorbance was plotted against the official American Oil Chemists’ Society’s FG method for samples that contained FG between 0.02 and 0.9%. Quadratic least squares regression for 31 samples had a correlation coefficient of r 2 =0.986 and a standard error of estimated FG of 0.032%.