Measurement of lipase activity in rubber ( Hevea brasiliensis ) seed | Zendy

Njoku O. U. | Zendy; Oogbu I. C. | Zendy; Eneh F. U. | Zendy

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Measurement of lipase activity in rubber ( Hevea brasiliensis ) seed

Author(s) -

Njoku O. U.,

Oogbu I. C.,

Eneh F. U.

Publication year - 1996

Publication title -

journal of the american oil chemists' society

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.512

H-Index - 117

eISSN - 1558-9331

pISSN - 0003-021X

DOI - 10.1007/bf02523512

Subject(s) - hevea brasiliensis , lipase , lipolysis , fatty acid , chemistry , food science , natural rubber , enzyme assay , oleic acid , extraction (chemistry) , enzyme , chromatography , biochemistry , adipose tissue , organic chemistry

Lipase activity in para rubber ( Hevea brasiliensis ) seed was measured by monitoring the release of free fatty acid by lipolysis of the endogenous lipid in a crushed sample of seed incubated at 37.5°C for 30 min. Free fatty acid was determined colorimetrically by a modified copper soap method. Fresh seeds showed the highest lipase activity. Drying the seeds at 60°C inactivated the enzyme. Drying of the seed at this temperature may be useful as a pretreatment for extraction of oil from the seed.

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