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Measurement of lipase activity in rubber ( Hevea brasiliensis ) seed
Author(s) -
Njoku O. U.,
Oogbu I. C.,
Eneh F. U.
Publication year - 1996
Publication title -
journal of the american oil chemists' society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.512
H-Index - 117
eISSN - 1558-9331
pISSN - 0003-021X
DOI - 10.1007/bf02523512
Subject(s) - hevea brasiliensis , lipase , lipolysis , fatty acid , chemistry , food science , natural rubber , enzyme assay , oleic acid , extraction (chemistry) , enzyme , chromatography , biochemistry , adipose tissue , organic chemistry
Lipase activity in para rubber ( Hevea brasiliensis ) seed was measured by monitoring the release of free fatty acid by lipolysis of the endogenous lipid in a crushed sample of seed incubated at 37.5°C for 30 min. Free fatty acid was determined colorimetrically by a modified copper soap method. Fresh seeds showed the highest lipase activity. Drying the seeds at 60°C inactivated the enzyme. Drying of the seed at this temperature may be useful as a pretreatment for extraction of oil from the seed.