Premium
Production of triglycerides enriched in long‐chain n‐3 polyunsaturated fatty acids from fish oil
Author(s) -
Moore Stephen R.,
McNeill Gerald P.
Publication year - 1996
Publication title -
journal of the american oil chemists' society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.512
H-Index - 117
eISSN - 1558-9331
pISSN - 0003-021X
DOI - 10.1007/bf02523504
Subject(s) - chemistry , glyceride , fish oil , hydrolysis , docosahexaenoic acid , lipase , polyunsaturated fatty acid , eicosapentaenoic acid , rhizomucor miehei , glycerol , fatty acid , chromatography , lipolysis , triacylglycerol lipase , food science , organic chemistry , biochemistry , fish <actinopterygii> , enzyme , biology , adipose tissue , fishery
Processes that combine enzymic and physical techniques have been studied for concentrating and separating eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) from fish oil. Candida rugosa lipase was used in hydrolysis reactions to concentrate these acids in the glyceride fraction. By controlling the degree of hydrolysis, two products have been obtained, one enriched in total n‐3(∼50%), the other enriched in DHA and depleted in EPA (DHA∼40%, EPA∼7%). The glyceride fraction from these reactions was recovered by evaporation and converted back to triglycerides by partial enzymic hydrolysis, followed by enzymic esterification. Both reactions were carried out with Rhizomucor miehei lipase. DHA‐depleted free fatty acids from a C. rugosa hydrolysis were fractionated to increase the EPA level (∼30%) and re‐esterified to triglycerides by reaction with glycerol and R. miehei .