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A novel compound, 12,13,17‐trihydroxy‐9( Z )‐Octadecenoic acid, from linoleic acid by a new microbial isolate Clavibacter sp. ALA2
Author(s) -
Hou Ching T.
Publication year - 1996
Publication title -
journal of the american oil chemists' society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.512
H-Index - 117
eISSN - 1558-9331
pISSN - 0003-021X
DOI - 10.1007/bf02523497
Subject(s) - linoleic acid , strain (injury) , chemistry , yield (engineering) , nuclear magnetic resonance spectroscopy , transformation (genetics) , fatty acid , chemical structure , biotransformation , mass spectrometry , stereochemistry , chromatography , organic chemistry , biochemistry , biology , materials science , enzyme , gene , anatomy , metallurgy
A novel compound, 12,13,17‐trihydroxy‐9( Z )‐octadecenoic acid (THOA), was produced from linoleic acid by microbial transformation at 25% yield. The newly isolated microbial strain that catalyzed this transformation was identified as Clavibacter sp. ALA2. The product was purified by high‐pressure liquid chromatography, and its structure was determined by 1 H and 13 C nuclear magnetic resonance, Fourier transform infrared, and mass spectroscopy. Maximum production of THOA was reached after 85 h of reaction. THOA was not further metabolized by strain ALA2. This is the first report on 12,13,17‐trihydroxy unsaturated fatty acid and its production by microbial transformation.