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Quantitative 31 P nuclear magnetic resonance analysis of the phospholipids of erythrocyte membranes using detergent
Author(s) -
NouriSorkhabi M. Hossein,
Wright Lesley C.,
Sullivan David R.,
Kuchel Philip W.
Publication year - 1996
Publication title -
lipids
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.601
H-Index - 120
eISSN - 1558-9307
pISSN - 0024-4201
DOI - 10.1007/bf02522893
Subject(s) - membrane , erythrocyte membrane , quantitative analysis (chemistry) , lipidology , chemistry , clinical chemistry , nuclear magnetic resonance spectroscopy , nuclear magnetic resonance , chromatography , biochemistry , physics , organic chemistry
31 P Nuclear magnetic resonance (NMR) spectra of human erythrocyte lysates dissolved in sodium cholate were acquired. The narrow resonances of phospholipids were mostly well resolved, allowing identification and accurate quantitative analysis of phospholipid classes of the erythrocyte membranes. The ether‐linked phosphatidylethanolamine components of the erythrocyte membranes were identified, based on the removal of plasmalogens by acidolysis and of diacyl phospholipid species by degradation using phospholipase A 1 . It was also shown that the introduction of double bonds on the acyl chains of phosphatidylcholine shifted the 31 P NMR resonances to lower frequencies. Quantitative analyses of phospholipids from the spectra were based on their apparent molar concentrations. The recoveries of phospholipids from erythrocytes were significantly higher than those using conventional extraction procedures.