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Sterol composition of Phaeodactylum tricornutum as influenced by growth temperature and light spectral quality
Author(s) -
Véron Benoît,
Billard Chantal,
Dauguet JeanClaude,
Hartmann MarieAndrée
Publication year - 1996
Publication title -
lipids
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.601
H-Index - 120
eISSN - 1558-9307
pISSN - 0024-4201
DOI - 10.1007/bf02522694
Subject(s) - sterol , phaeodactylum tricornutum , chemistry , composition (language) , chromatography , food science , algae , biochemistry , botany , biology , cholesterol , linguistics , philosophy
In a detailed sterol analysis of the marine diatom Phaeodactylum tricornutum , free sterols as well as esterified and glycosylated conjugates were found. When the alga was grown under standard conditions (i.e., at 13°C under white light), 64% of total sterols were steryl glycosides. In all sterol classes, except steryl esters, (24S)‐24‐methylcholesta‐5, 22E‐ ‐dien‐3β‐ol (epibrassicasterol) was the major (80 to 99%) sterol component. Eight other sterols were identified. Growth under different light spectral quality (red, blue, yellow, and green) at 13 and 23°C was examined. At 23°C, a dramatic decrease in total sterol content was observed, especially under blue light. The distribution of sterols between free and conjugated forms as well as sterol profile inside each class was found to be strongly dependent on the light spectral quality at both temperatures.