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An improved method for rapid analysis of the fatty acids of glycerolipids
Author(s) -
Ichihara Ken’ichi,
Shibahara Akira,
Yamamoto Kohei,
Nakayama Takao
Publication year - 1996
Publication title -
lipids
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.601
H-Index - 120
eISSN - 1558-9307
pISSN - 0024-4201
DOI - 10.1007/bf02522648
Subject(s) - transesterification , chemistry , hexane , chromatography , fish oil , linolenate , fatty acid methyl ester , fatty acid , gas chromatography , lipidology , elution , organic chemistry , fish <actinopterygii> , catalysis , biodiesel , biology , fishery
An improved rapid procedure to determine the fatty acid composition of glycerolipids is described. The procedure includes KOH‐catalyzed transesterification and high‐speed gas chromatography. Glycerolipids (20–40 mg) were mixed with 2 mL of hexane and 0.2 mL of 2 M methanolic KOH at room temperature for 1–2 min. The fatty acid methyl esters in the hexane layer were analyzed by gas chromatography on 10% SP‐2340 at 240°C. Methyl linolenate and docosahexaenoate eluted within 2 and 5 min, respectively. Analysis was thus completed with 5 min for common vegetable oils and 8 min for fish oils.