An improved method for rapid analysis of the fatty acids of glycerolipids | Zendy

Ichihara Ken’ichi | Zendy; Shibahara Akira | Zendy; Yamamoto Kohei | Zendy; Nakayama Takao | Zendy

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An improved method for rapid analysis of the fatty acids of glycerolipids

Author(s) -

Ichihara Ken’ichi,

Shibahara Akira,

Yamamoto Kohei,

Nakayama Takao

Publication year - 1996

Publication title -

lipids

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.601

H-Index - 120

eISSN - 1558-9307

pISSN - 0024-4201

DOI - 10.1007/bf02522648

Subject(s) - transesterification , chemistry , hexane , chromatography , fish oil , linolenate , fatty acid methyl ester , fatty acid , gas chromatography , lipidology , elution , organic chemistry , fish <actinopterygii> , catalysis , biodiesel , biology , fishery

An improved rapid procedure to determine the fatty acid composition of glycerolipids is described. The procedure includes KOH‐catalyzed transesterification and high‐speed gas chromatography. Glycerolipids (20–40 mg) were mixed with 2 mL of hexane and 0.2 mL of 2 M methanolic KOH at room temperature for 1–2 min. The fatty acid methyl esters in the hexane layer were analyzed by gas chromatography on 10% SP‐2340 at 240°C. Methyl linolenate and docosahexaenoate eluted within 2 and 5 min, respectively. Analysis was thus completed with 5 min for common vegetable oils and 8 min for fish oils.

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