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Changes in high density lipoprotein subfraction lipids during neutral lipid transfer in healthy subjects and in patients with insulin‐dependent diabetes mellitus
Author(s) -
Ritter Mary C.,
Bagdade John D.
Publication year - 1996
Publication title -
lipids
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.601
H-Index - 120
eISSN - 1558-9307
pISSN - 0024-4201
DOI - 10.1007/bf02522403
Subject(s) - medicine , endocrinology , very low density lipoprotein , cholesterylester transfer protein , chemistry , lipoprotein , lipidology , diabetes mellitus , cholesteryl ester , high density lipoprotein , clinical chemistry , triglyceride , apolipoprotein b , intermediate density lipoprotein , low density lipoprotein , blood lipids , cholesterol
While it is known that the transfer of cholesteryl ester (CE) from high density lipoprotein (HDL) to the apo B‐containing lipoproteins is increased in patients with diabetes, the extent to which the various lipoprotein fractions engage in neutral lipid exchange and the magnitude to which triglyceride (TG) is translocated is not known. To examine in greater detail neutral lipid net mass transfer in diabetes, the HDL subfractions and the apo B‐containing lipoproteins were separated, and the net mass transfer of CE and TG was compared to that of control subjects. In both groups, bidirectional transfer of CE from HDL 3 to very low density lipoprotein (VLDL) + low density lipoprotein (LDL) and of TG from VLDL+LDL to HDL 3 , took place, but this process was significantly greater ( P <.01) in insulin‐dependent diabetes mellitus (IDDM). In contrast, CE and TG accumulated in HDL 2 to a similar degree in normal and IDDM subjects. In recombination experiments with each of the apo B‐containing lipoproteins, IDDM VLDL had a greater capacity to facilitate the exchange of core lipids from both IDDM and control HDL 3 : on the other hand, LDL from IDDM and control subjects both donated TG and CE to HDL 2 and affected little change in HDL 3 . These findings indicate that all the major plasma fractions normally participate in the trafficking of CE and TG among the lipoproteins during neutral lipid transfer and show that the principal perturbation in cholesteryl ester transfer in IDDM involves altered interaction between VLDL and the HDL 3 subfraction.