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Morphological and functional changes after portal vein occlusion in rats
Author(s) -
Tanaka Hiromu,
Ikeda Kazuo,
Matsuyama Mitsuharu,
Kubo Shoji,
Hirohashi Kazuhiro,
Kinoshita Hiroaki
Publication year - 1997
Publication title -
journal of hepato‐biliary‐pancreatic surgery
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.63
H-Index - 60
eISSN - 1868-6982
pISSN - 0944-1166
DOI - 10.1007/bf02488967
Subject(s) - ornithine decarboxylase , hepatectomy , occlusion , medicine , dna synthesis , cirrhosis , liver regeneration , embolization , right gastric vein , indocyanine green , pathology , endocrinology , portal venous pressure , chemistry , biology , portal hypertension , surgery , regeneration (biology) , dna , biochemistry , resection , enzyme , microbiology and biotechnology
We investigated morphological and functional changes after portal vein occlusion in rats. Portal branches for the median and left lateral lobes were ligated, after which the lobes were examined micromorphologically. After embolization of the same branches, regenerative capacity was evaluated in normal livers and in livers with CCl 4 ‐induced cirrhosis. Indocyanine green elimination, antithrombin III activity, and Kupffer cell density were also investigated. In another set of rats, the embolized lobes were resected 0, 2, 4, or 7 days after portal vein embolization (PVE), and endotoxin was injected intravenously 48h after each hepatectomy. In the ligated lobes, apoptotic hepatocytes were detected mainly around a widespread necrotic area on day 2, and among normal hepatocytes on day 7. In the nonembolized livers of control rats, increases were noted in liver weight, ornithine decarboxylase (ODC) activity, DNA synthesis, and mitosis of hepatocytes. In the cirrhotic livers, ODC activity was stimulated in a fashion similar to that seen in control liver, but DNA synthesis and weight change was delayed, although not significantly. On days 2, 4, 7, and 14 PVE, Kupffer cell density was about twice that seen in rats before PVE. Endotoxin‐induced liver injury was slight if the rats had received PVE 4–7 days before the hepatectomy.