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Biochemical and metabolic assessments of liver graft preserved with oncotic oxygen carrier
Author(s) -
Tanaka JunIchi,
Takino Hiroaki,
Malchesky Paul S.,
Koyama Kenji
Publication year - 1994
Publication title -
journal of hepato‐biliary‐pancreatic surgery
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.63
H-Index - 60
eISSN - 1868-6982
pISSN - 0944-1166
DOI - 10.1007/bf02391102
Subject(s) - oncotic pressure , viaspan , oxygen , machine perfusion , perfusion , adenosine triphosphate , chemistry , hemoglobin , cold storage , medicine , biochemistry , surgery , transplantation , biology , liver transplantation , albumin , organic chemistry , horticulture
The effect of an oxygen‐carrying agent, as a component of the preservation solution, on the biochemical viability and energy metabolism of the graft in liver preservation was evaluated. Two preservation methods, simple hypothermic storage and continuous machine perfusion, both performed at 4°C for 48 h, were studied in male Lewis rats. Pyridoxylated hemoglobin‐polyoxyethylene conjugate (PHP) was used as the oncotic oxygen carrier. University of Wisconsin (UW) solution served as a control solution. The test solution (PHP+UW) was a 1:1 mixture of PHP and UW solutions with 4.0g% of hemoglobin. We observed that oxygen consumption during perfusion was significantly higher in the PHP+UW than in the UW group. Hepatic mitochondrial function and tissue adenosine‐5′‐triphosphate (ATP) levels were better preserved in the PHP+UW than in the UW group, and were also better preserved in continuous machine perfusion than in simple hypothermic storage. No significant differences were observed in perfusate biochemical parameters. These results indicate that the incorporation of an oxygen‐carrying agent into preservation solution provides significantly better oxygen delivery to liver grafts, and may improve hepatic viability.