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Glycine transport by cultured skin fibroblasts from a patient with isolated hyperglycinuria
Author(s) -
Fénéant M.,
Moatti N.,
Lemonnier F.,
Maccario J.,
Gautier M.,
Charpentier C.,
Lemonnier A.
Publication year - 1980
Publication title -
journal of inherited metabolic disease
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.462
H-Index - 102
eISSN - 1573-2665
pISSN - 0141-8955
DOI - 10.1007/bf02312539
Subject(s) - glycine , fibroblast , cell culture , significant difference , microbiology and biotechnology , chemistry , medicine , biology , biochemistry , genetics , amino acid
Glycine transport is studied in cultured skin fibroblasts from a patient with isolated hyperglycinuria and from five normal subjects. Fibroblasts from the patient take up glycine less well than do cell lines from controls. Kinetic studies are consistent with a single transport system in the patient's and controls' cell lines. V max value in the hyperglycinuric lines is normal, but the apparent affinity is always reduced as opposed to those of four different control lines separately tested. Statistical analysis shows significant difference between K m values.