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The 4‐methylumbelliferone sulphate sulphatases of human tears
Author(s) -
Jordan T. W.,
Heiss F.,
Monk B.,
Nicholls J.
Publication year - 1980
Publication title -
journal of inherited metabolic disease
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.462
H-Index - 102
eISSN - 1573-2665
pISSN - 0141-8955
DOI - 10.1007/bf02312523
Subject(s) - tears , metachromatic leukodystrophy , isoelectric focusing , enzyme , chemistry , isoelectric point , biochemistry , enzyme assay , population , normal population , biology , medicine , immunology , environmental health
The properties of human tear 4‐methylumbelliferone sulphate sulphatase (EC 3.1.6.1) have been investigated. More than 80% of the enzyme activity behaved as the acidic A isoenzyme on isoelectric focusing and DEAE‐cellulose ion exchange chromatography. The distribution of enzyme activity in a normal population has been investigated. The least interindividual variation was seen when enzyme activities were calculated as units per mg tears. The α‐galactosidase. Β‐ N ‐acetylglucosaminidase and sulphatase enzyme activities in human tears varied independently of each other. The use of tear sulphatases for the detection of metachromatic leukodystrophy (McKusick 24980) is discussed.