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Synthesis of conjugates for production of an antibody to ortho ‐hydroxyphenylacetic acid
Author(s) -
Tyfield L. A.,
Ismail A. A. A.,
Holton J. B.,
McOmie J. F. W.
Publication year - 1981
Publication title -
journal of inherited metabolic disease
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.462
H-Index - 102
eISSN - 1573-2665
pISSN - 0141-8955
DOI - 10.1007/bf02263623
Subject(s) - hapten , conjugate , lysine , diazo , chemistry , radioimmunoassay , peptide , amino acid analysis , bovine serum albumin , antibody , amino acid , biochemistry , amide , stereochemistry , biology , organic chemistry , immunology , mathematical analysis , mathematics
Raising antibodies to a small, chemically simple molecule is difficult. Attempts have been made to develop a simple, sensitive and specific radioimmunoassay for the phenolic compound, o ‐hydroxyphenylacetic acid (OHPAA) (mol. wt. 152). To date, five different immunogens have been synthesized. Two conjugates of OHPAA were linked to bovine serum albumin (BSA) and injected into rabbits and three were linked to poly‐ l ‐lysine for injection into Strain 2 PLL+guinea‐pigs. The peptide linkage in one was via the carboxyl group to the ε‐amino lysine residues of BSA. The other four were linked in a diazo linkage to the carrier polypeptide through an amino group which had been added to the hapten molecule. So far, antibodies specific to the hapten portion of any of the conjugates have not been obtained. Further conjugates containing methoxyphenylacetic acid and a succinate bridge are being prepared.