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Estimation of energy metabolism in human skeletal muscle homogenate as a diagnostic aid
Author(s) -
Ruitenbeek W.,
Sengers R. C. A.,
Trijbels J. M. F.,
Stadhouders A. M.,
Janssen A. J. M.
Publication year - 1981
Publication title -
journal of inherited metabolic disease
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.462
H-Index - 102
eISSN - 1573-2665
pISSN - 0141-8955
DOI - 10.1007/bf02263606
Subject(s) - skeletal muscle , creatine , metabolite , human muscle , enzyme , energy metabolism , metabolism , biochemistry , medicine , incubation , endocrinology , biology , chemistry
Abstract A method is presented for the in vitro testing of the energy metabolism of human skeletal muscle. The levels of creatine phosphate, ATP, ADP and AMP, which are defined by the activity of many enzyme systems, are measured after incubation of the muscle homogenate with various substrates in the presence or absence of specific inhibitors. Detection of abnormal metabolite concentrations is important for diagnosis of patients with myopathic syndromes.