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Effects of econazole, fungizone and pimafucin on cell growth, lysosomal enzyme activity and sulphate metabolism of cultured human skin fibroblasts and amniotic fluid cells
Author(s) -
Butterworth J.
Publication year - 1982
Publication title -
journal of inherited metabolic disease
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.462
H-Index - 102
eISSN - 1573-2665
pISSN - 0141-8955
DOI - 10.1007/bf02179137
Subject(s) - econazole , amniotic fluid , enzyme , cell culture , biochemistry , human skin , biology , cell growth , cell , microbiology and biotechnology , chemistry , fetus , antifungal , miconazole , pregnancy , genetics
Human skin fibroblasts and amniotic fluid cells showed sensitivity to antifungal agents in the order Econazole < Pimafucin < Fungizone, with the last initially reducing cell growth 10‐20%, even at the level recommended for cell culture. The activities of the lysosomal enzymes α‐ l ‐fucosidase, β‐ d ‐glucuronidase and α‐ d ‐mannosidase were unaffected, even by high concentrations of all three antifungal agents. Sulphate incorporation by cultured fibroblasts was increased by removal of sulphate salts and Crystamycin (contains streptomycin sulphate) from the culture medium. Sulphate incorporation into and degradation from macromolecules was only slightly reduced by antifungal agents. Human skin fibroblasts and amniotic fluid cells can be cultured long term in the presence of Econazole or Fungizone and used for lysosomal enzyme assay and sulphate kinetic studies.