Serum-induced stimulation of snRNA synthesis in mouse 3T3 fibroblasts | Zendy

J.M. Tiercy | Zendy; Robert Weil | Zendy

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Serum-induced stimulation of snRNA synthesis in mouse 3T3 fibroblasts

Author(s) -

J.M. Tiercy,

Robert Weil

Publication year - 1985

Publication title -

experientia

Language(s) - English

Resource type - Journals

ISSN - 0014-4754

DOI - 10.1007/bf02005887

Subject(s) - rna , ethidium bromide , uridine , small nuclear rna , microbiology and biotechnology , stimulation , 3t3 cells , ribosomal rna , biology , staining , polyacrylamide , biochemistry , chemistry , genetics , dna , rna dependent rna polymerase , endocrinology , transfection , gene

Small nuclear RNAs (snRNAs) from quiescent and serum-stimulated 3T3 cultures, labeled with [3H]uridine [( 3H]U), were electrophoresed in polyacrylamide-urea slab gels and revealed by staining with ethidium bromide and by fluorography. Judged by labeling with [3H]U, synthesis of 7S and U1-U6 RNAs was very low or absent in quiescent cultures. The serum-induced transition of 3T3 cells from a resting to a growing state was accompanied by an early, apparently sequential stimulation of snRNA synthesis; stimulated synthesis of 7S, U1, U2, U3, U4 and U6 RNAs coincided in time with serum-induced stimulation of 45S pre-ribosomal RNA (pre-rRNA) and heterogeneous nuclear RNA (hnRNA) synthesis.

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