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Inability of second trimester human amniotic fluid cell cultures to aromatize C 19 ‐steroids
Author(s) -
O'Shannessy D. J.,
Renwick A. G. C.
Publication year - 1985
Publication title -
journal of inherited metabolic disease
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.462
H-Index - 102
eISSN - 1573-2665
pISSN - 0141-8955
DOI - 10.1007/bf01805481
Subject(s) - amniotic fluid , radioimmunoassay , endocrinology , medicine , aromatase , amniocentesis , chemistry , diethylstilbestrol , in vitro , fetus , andrology , biology , estrogen , biochemistry , pregnancy , prenatal diagnosis , genetics , cancer , breast cancer
Amniotic fluid (AF) and fibroblast (F) cell types, derived from human amniotic fluid by amniocentesis in the second trimester, were cultured using media of varying composition and their ability to transform C 19 ‐steroids to oestrogens was investigated by fluorimetry, radioimmunoassay, tritium release and measurement of cytochrome P 450 . Although the cells were shown to be metabolically active by the presence of mitotic figures, the incorporation of tritiated leucine into protein and the production of the β‐subunit of hCG, no evidence of aromatization was obtained despite the inclusion in the medium of a number of steroids known to be transformed to oestrogens in several in vivo and in vitro preparations. The addition of reported stimulants of aromatase activity, viz. hCG, FSH, diethylstilbestrol, prostaglandins E 2 and F 2α and dibutyryl cyclic AMP, was without effect.