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Confocal scanning laser microscopy of mitochondria: a possible tool in the diagnosis of mitochondrial disorders
Author(s) -
Ruiters M. H. J.,
Spronsen E. A.,
Skjeldal O. H.,
Stromme P.,
Scholte H. R.,
Pzyrembel H.,
Smit G. P. A.,
Ruitenbeek W.,
Agsteribbe E.
Publication year - 1991
Publication title -
journal of inherited metabolic disease
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.462
H-Index - 102
eISSN - 1573-2665
pISSN - 0141-8955
DOI - 10.1007/bf01804387
Subject(s) - mitochondrion , confocal , mitochondrial myopathy , confocal microscopy , confocal laser scanning microscopy , microscopy , pathology , staining , mitochondrial disease , biology , chemistry , microbiology and biotechnology , biophysics , medicine , mitochondrial dna , biochemistry , optics , physics , gene
Summary This paper describes a non‐invasive method for the study of mitochondrial morphology in cultured human skin fibroblasts by confocal scanning laser microscopy after staining the mitochondria with 2‐[4‐(dimethylaminostyryl]‐1‐methylpyridinium iodide. This method is applied to compare mitochondria in fibroblasts from healthy individuals and from patients with mitochondrial myopathy. In most cases there is a striking swelling of the patient's mitochondria and a loss of fine structure. The results with respect to the potential of this method as a diagnostic tool are discussed.