Confocal scanning laser microscopy of mitochondria: a possible tool in the diagnosis of mitochondrial disorders | Zendy

Ruiters M. H. J. | Zendy; Spronsen E. A. | Zendy; Skjeldal O. H. | Zendy; Stromme P. | Zendy; Scholte H. R. | Zendy; Pzyrembel H. | Zendy; Smit G. P. A. | Zendy; Ruitenbeek W. | Zendy; Agsteribbe E. | Zendy

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Confocal scanning laser microscopy of mitochondria: a possible tool in the diagnosis of mitochondrial disorders

Author(s) -

Ruiters M. H. J.,

Spronsen E. A.,

Skjeldal O. H.,

Stromme P.,

Scholte H. R.,

Pzyrembel H.,

Smit G. P. A.,

Ruitenbeek W.,

Agsteribbe E.

Publication year - 1991

Publication title -

journal of inherited metabolic disease

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.462

H-Index - 102

eISSN - 1573-2665

pISSN - 0141-8955

DOI - 10.1007/bf01804387

Subject(s) - mitochondrion , confocal , mitochondrial myopathy , confocal microscopy , confocal laser scanning microscopy , microscopy , pathology , staining , mitochondrial disease , biology , chemistry , microbiology and biotechnology , biophysics , medicine , mitochondrial dna , biochemistry , optics , physics , gene

Summary This paper describes a non‐invasive method for the study of mitochondrial morphology in cultured human skin fibroblasts by confocal scanning laser microscopy after staining the mitochondria with 2‐[4‐(dimethylaminostyryl]‐1‐methylpyridinium iodide. This method is applied to compare mitochondria in fibroblasts from healthy individuals and from patients with mitochondrial myopathy. In most cases there is a striking swelling of the patient's mitochondria and a loss of fine structure. The results with respect to the potential of this method as a diagnostic tool are discussed.

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