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Streptozotocin‐induced diabetes is not a model for methylmalonic acidaemia
Author(s) -
Bailey L. B.,
Molloy A.,
Scott J.,
Rice D.
Publication year - 1989
Publication title -
journal of inherited metabolic disease
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.462
H-Index - 102
eISSN - 1573-2665
pISSN - 0141-8955
DOI - 10.1007/bf01802038
Subject(s) - glycosuria , methylmalonic acid , streptozotocin , diabetes mellitus , endocrinology , medicine , urine , urinary system , vitamin , vitamin b12 , chemistry
Summary The streptozotocin‐treated diabetic rat was not found to be a suitable animal model for methylmalonic acidaemia as previously described. Urinary methylmalonic acid (MMA) was measured in adult Wistar rats prior to and following injection of streptozotocin (40 mg/kg). Plasma and tissue MMA levels were measured following the induction of diabetes and compared with data from control rats. MMA levels were determined by a gas chromatographic‐mass spectrometric method (McMurray et al. , 1986). Diabetes was confirmed by the 10 × increase in 24h urine volume; glycosuria; glycaemia; and weight loss. Urinary MMA excreted did not change during the 11 week diabetic period and there was no difference between the pre‐ and post‐diabetic phases ( p <0.05). Plasma and tissue MMA concentrations were not elevated in this diabetic animal model. Also in contrast to earlier reports, the vitamin B 12 levels of the diabetic rats were not elevated compared to controls ( p <0.05).