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Determination of serum apolipoprotein B by competitive enzyme‐linked immunoassay
Author(s) -
Reisinger H.,
Strobl W.,
Hubrich B.,
Widhalm K.
Publication year - 1991
Publication title -
journal of inherited metabolic disease
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.462
H-Index - 102
eISSN - 1573-2665
pISSN - 0141-8955
DOI - 10.1007/bf01800598
Subject(s) - apolipoprotein b , radial immunodiffusion , immunoassay , immunodiffusion , chemistry , enzyme , coefficient of variation , medicine , cholesterol , endocrinology , chromatography , antibody , immunology , biochemistry
Summary An enzyme‐linked immunoassay was used to determine apolipoprotein B (apoB) in serum samples from 104 healthy adults aged 18–26 years. The method was compared to radial immunodiffusion (RID). Mean apoB values were 103±30 mg/dl and 105±31 mg/dl respectively. The correlation coefficient for apoB between the two methods was r =0.70, p <0.001; the correlation coefficient between LDL‐cholesterol and apoB measured by the ELISA was r =0.73, p <0.001. This ELISA technique seems to be an appropriate method for measuring apoB in plasma, in the clinical laboratory and for detecting individuals with hyperapobetalipoproteinaemia.