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Culture of galactosaemic fibroblasts in the presence of galactose: Effect of inosine
Author(s) -
Pourci M. L.,
Mangeot M.,
Soni T.,
Lemonnier A.
Publication year - 1990
Publication title -
journal of inherited metabolic disease
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.462
H-Index - 102
eISSN - 1573-2665
pISSN - 0141-8955
DOI - 10.1007/bf01800205
Subject(s) - galactose , hexose , fibroblast , in vitro , inosine , biochemistry , fetus , cell culture , biology , reversion , fetal bovine serum , cell , metabolism , chemically defined medium , fructose , chemistry , phenotype , enzyme , pregnancy , genetics , gene
Summary Fibroblasts from three galactosaemics had no galactose‐1‐phosphate uridyltransferase (GALT) activity. These fibroblast cells were cultured in different media supplemented with dialysed fetal calf serum. Galactosaemic and control cell strains stopped growing in hexose‐free medium. In glucose‐free medium containing galactose, galatosaemic cells, in contrast to control cells, stopped growing after two days and died. In the same medium supplemented with inosine, they exhibited the same growth pattern as the control cell strains although in the presence of high concentrations of galactose‐1‐phosphate (Gal‐1‐P). These findings indicated that the glucose‐free medium containing galactose supplemented with dialysed fetal calf serum and inosine, as a ribose donor, was appropriate for further in vitro investigations of galactose metabolism in galactosaemic cells.