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Cell genetic studies on propionyl‐CoA carboxylase deficient cell lines
Author(s) -
Leeuwen G. H.,
Vrieze G.,
Gimpel J. A.,
Huisjes H. J.,
Hommes F. A.
Publication year - 1982
Publication title -
journal of inherited metabolic disease
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.462
H-Index - 102
eISSN - 1573-2665
pISSN - 0141-8955
DOI - 10.1007/bf01800004
Subject(s) - complementation , cell culture , cell fusion , pyruvate carboxylase , biochemistry , trichloroacetic acid , heterokaryon , mutant , fibroblast , cell , propionate , microbiology and biotechnology , biology , chemistry , genetics , enzyme , gene
Thirteen propionyl‐CoA carboxylase (PCC)‐deficient fibroblast lines were analysed for complementation by examining the incorporation of [ 14 C]propionate into trichloroacetic‐acid insoluble cellular macromolecules of polyethylene‐glycol‐dimethyl‐sulphoxide induced heterokaryons formed by fusion of mutant cell lines. Corrections for variations in the rate of protein synthesis of the heterokaryons and individual cell lines was made by measuring the incorporation of [ 3 H]lysine, added simultaneously with the labelled propionate. Two distinct complementation groups were found in this sample of PCC‐deficient cell lines. Evidence for intragenic complementation was not obtained.