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Stable‐isotope selected‐ion monitoring quantification of methylmalonic acid in dried filter‐paper urine samples
Author(s) -
Parnet J. M.,
Divry P.,
VianeySaban C.,
Mathieu M.
Publication year - 1996
Publication title -
journal of inherited metabolic disease
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.462
H-Index - 102
eISSN - 1573-2665
pISSN - 0141-8955
DOI - 10.1007/bf01799839
Subject(s) - urine , methylmalonic acid , chromatography , chemistry , creatinine , methylmalonic acidemia , mass spectrometry , urea , methylmalonic aciduria , filter paper , biochemistry , medicine , homocysteine
Summary Urea and creatinine were measured by Kodak Ektachem methods and methylmalonic acid by stable‐isotope gas chromatography—mass spectrometry in 24 urine samples from patients with methylmalonic acidaemia. For each sample analyses were performed both directly on the liquid urine and on an aliquot which had been blotted onto filter paper and dried. There was good correlation between the methylmalonate/creatinine ratios in liquid and dried urine ( r 2 =0.983). Urine dried on filter paper can be used advantageously for monitoring treatment in patients with this disorder.