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Removal of branched‐chain amino acids and α ‐ketoisocaproate by haemofiltration and haemodiafiltration
Author(s) -
Gouyon J. B.,
Semama D.,
Prévot A.,
Desgres J.
Publication year - 1996
Publication title -
journal of inherited metabolic disease
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.462
H-Index - 102
eISSN - 1573-2665
pISSN - 0141-8955
DOI - 10.1007/bf01799834
Subject(s) - valine , isoleucine , leucine , amino acid , ultrafiltration (renal) , chemistry , hemofiltration , medicine , maple syrup urine disease , chromatography , endocrinology , biochemistry , biology , hemodialysis
Summary Venovenous haemofiltration (VVHF) and haemodiafiltration (VVHDF) were performed with a neonatal haemo(dia)filter (Miniflow 10, Hospal) on 8 anaesthetized rabbits infused with branched‐chain amino acids (leucine, isoleucine and valine) and α ‐ketoisocaproate. The branched‐chain amino acids (BCAA) and α ‐ketoisocaproate blood levels were close to those previously observed in neonates with maple syrup urine disease when extracorporeal blood purification was required. VVHF and VVHDF performances were assessed with two different blood flows (Q ˙ b = 8 . 3 and 16.6 ml/min). VVHDF was performed with four dialysate flow rates (Q ˙ d = 0 . 5 , 1 . 0 , 2 . 0 and 3.0 L/h). Within each period, clearances of the three BCAA were strictly similar. BCAA clearances obtained by VVHF were similar to ultrafiltration rates (respectively, 0.78±0.14 and 1.79±0.28 ml/min at high and low Q ˙ b ; p <0.05). The α ‐ketoisocaproate clearances obtained by VVHF were 0.39±0.17 and 0.92±0.43 ml/min at low and high Q ˙ b (not significantly different). Whatever the Q ˙ d value, the VVHDF procedures always allowed higher BCAA and α ‐ketoisocaproate clearances as compared with the corresponding VVHF period with similar Q ˙ b . BCAA clearances obtained by VVHDF with a 0.5 L/h dialysate flow were 4.1±0.5 and 5.4±0.5 mL/min at low and high Q ˙ b , respectively. The concurrent α ‐ketoisocaproate clearances were 2.5±0.8 and 2.9±1.0 ml/min.