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Review: Preimplantation diagnosis of inherited disease
Author(s) -
Lissens W.,
Sermon K.,
Staessen C.,
Van Assche E.,
Janssenswillen C.,
Joris H.,
Van Steirteghem A.,
Liebaers I.
Publication year - 1996
Publication title -
journal of inherited metabolic disease
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.462
H-Index - 102
eISSN - 1573-2665
pISSN - 0141-8955
DOI - 10.1007/bf01799159
Subject(s) - preimplantation genetic diagnosis , blastomere , prenatal diagnosis , disease , in vitro fertilisation , embryo , embryo transfer , pregnancy , medicine , fetus , biology , obstetrics , genetics , pathology , embryogenesis
Summary Preimplantation diagnosis of inherited diseases has become possible with the techniques of in vitro fertilization, blastomere biopsy of the 6‐ to 10‐cell embryo and DNA analysis of the single blastomeres. Disease‐free embryos are selected for transfer to the uterus, thereby avoiding the need for termination of a fetus diagnosed as affected in prenatal diagnosis in the first or early‐second trimester of pregnancy. The genetic indications for preimplantation diagnosis are theoretically the same as for prenatal diagnosis, but the defects must be detectable by the polymerase chain reaction. For X‐linked recessive diseases, fluorescence in situ hybridization can be used as an alternative for the selection of female embryos. So far almost 40 healthy children have been born worldwide after preimplantation diagnosis for genetic disease. The possibilities and limitations of preimplantation diagnosis, especially in prevention of inherited disease, are discussed in this review.