Open AccessPhotodynamic DNA-breaking activity of serum from patients with various photosensitivity dermatoses
Author(s) -
Hideo Hashizume,
Y. Tokura,
Tomozoh Oku,
Yoshihisa Iwamoto,
Masahiro Takigawa
Publication year - 1995
Publication title -
archives of dermatological research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.776
H-Index - 80
eISSN - 1432-069X
pISSN - 0340-3696
DOI - 10.1007/bf00374081
Subject(s) - photosensitivity , erythropoietic protoporphyria , phototoxicity , dermatology , porphyria cutanea tarda , medicine , porphyria , photodermatosis , dna damage , chemistry , dna , photochemistry , protoporphyrin , biochemistry , porphyrin , physics , xeroderma pigmentosum , quantum mechanics , in vitro
Various drugs and chemicals break the DNA strand under ultraviolet irradiation. This study aimed to clarify the DNA-breaking activity (DBA) of serum from 39 patients with various photosensitivity disorders and that from eight normal subjects. A mixture of serum and circular plasmid DNA was exposed to longwave ultraviolet radiation, and the photoinduced cleavage of plasmid DNA was examined by electrophoretic analysis. DBA was found in serum from patients with erythropoietic protoporphyria (2 of 2), drug-induced photosensitivity (3 of 5), chronic actinic dermatitis (1 of 12) and hydroa vacciniforme (1 of 1). DBA was not found in serum from patients with porphyria cutanea tarda, collagen diseases with photosensitivity, papulovesicular light eruption or pellagra. The inhibition profile of DBA by active oxygen scavengers was different between afloqualone- and tetracycline-induced photosensitivity and chronic actinic dermatitis. The present method was useful for the detection of serum phototoxicity and the investigation of the pathomechanisms of photosensitivity.
Accelerating Research
Robert Robinson Avenue,
Oxford Science Park, Oxford
OX4 4GP, United Kingdom
Address
John Eccles HouseRobert Robinson Avenue,
Oxford Science Park, Oxford
OX4 4GP, United Kingdom