Replication of pSC101: effects of mutations in the E. coli DNA binding protein IHF | Zendy

Pascal Gamas | Zendy; Anne-Catherine Burger | Zendy; Glen Churchward | Zendy; L. Caro | Zendy; David J. Galas | Zendy; Michaël Chandler | Zendy

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Replication of pSC101: effects of mutations in the E. coli DNA binding protein IHF

Author(s) -

Pascal Gamas,

Anne-Catherine Burger,

Glen Churchward,

L. Caro,

David J. Galas,

Michaël Chandler

Publication year - 1986

Publication title -

mgg molecular and general genetics

Language(s) - English

Resource type - Journals

eISSN - 1432-1874

pISSN - 0026-8925

DOI - 10.1007/bf00330192

Subject(s) - dnaa , biology , plasmid , dna replication , dna , origin recognition complex , origin of replication , dna binding protein , genetics , microbiology and biotechnology , gene , eukaryotic dna replication , transcription factor

We have shown that the plasmid pSC101 is unable to be maintained in strains of E. coli carrying deletions in the genes himA and hip which specify the pleitropic heterodimeric DNA binding protein, IHF. We show that this effect is not due to a modulation of the expression of the pSC101 RepA protein, required for replication of the plasmid. Inspection of the DNA sequence of the essential replication region of pSC101 reveals the presence of a site, located between the DnaA binding-site and that of RepA, which shows extensive homology with the consensus IHF binding site. The proximity of the sites suggests that these three proteins, IHF, DnaA, and RepA may interact in generating a specific DNA structure required for initiation of pSC101 replication.

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